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Specialists (diabetologists and endocrinologists) made up almost half of the sample and primary care physicians were relatively equally divided among internal medicine, general medicine and family practice.
This is followed by addition of sample and primary antibody and after overnight incubation the amount of bound antibody is made visible using a peroxidase-labeled secondary antibody and a chromogenic peroxidase substrate.
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An additional assessment with 780 randomly stratified samples and primary and alternative calls in the reference data to account for ambiguity indicated 83.4% overall accuracy (Kappa of 0.80).
Processing of biopsy samples and primary keratinocyte culture was performed as described [16].
Control samples and primary cells were obtained from ancestry-, sex- and age-matched healthy individuals.
DG managed the samples and primary data at TMMC, and helped to interpret the results and draft the manuscript.
We also show that using a combination of Braf and p300 expression, it is possible to separate nevi and melanoma samples, and primary and metastatic melanoma samples.
The high expression levels of B7-H4 in both primary EMCAR (90%% of samples) and primary US (92 % of samples) make this an attractive candidate for uterine cancer therapy.
This finding was further supported by detection of the identical BAP1 mutations in tumor xenografts, and the corresponding primary tumor samples and primary cell cultures (Table 3).
The set of metastatic samples and primary tumors analyzed with the CodeLink platform were extracted using the RNeasy kit (Qiagen, San Diego, CA).
To compare ASPM protein levels in established cancer cell lines, benign samples and primary cultures, a slot blotting technique was applied.
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