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In short, after we treated cells with glutamine for 24 h, 90 μl/well of the ATP reaction mix was added into the sample and mixed gently, and then incubated for 10 20 min in the dark at room temperature.
The chromatograms of sample and mixed standard were shown in Fig. 8.
Following vortex mixing for 5 min, buffer AL (200 µl) was added to the sample and mixed.
The main limitation of this pilot study is the small sample, and mixed tumour type, both in terms of tumour staging and primary sites.
After a 24-h period for radioactive decay, two 100-μL aliquots were taken from each sample and mixed with soybean casein digest medium and fluid thioglycollate medium, respectively, and incubated for 14 days at 37 °C.
gDNA Wipeout buffer (7 x) was added to each sample and mixed by pipetting.
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36 The generalisability of these findings is limited by the small sample size and mixed sample of children with anxiety alone and ADHD/anxiety.
random route sampling and mixed frame telephone interviews, are sufficient.
Five hundred microliters of 100-mM anhydrous disodium hydrogen phosphate was added to all samples and mixed by vortex.
The mode itself is the means by which information has been collected - in person, live caller, voice recognition call, internet sampling, and mixed landline / cell live caller polls.
We thawed frozen samples and mixed them with 0.1 vol 5.5% Triton X-100.
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