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The standard weight (5 kg) was placed on the top of the sample and maintained for 2 min.
This is essentially what happens when a fungus is purified from an environmental sample and maintained in a laboratory environment.
As described previously [ 10], immediately after shearing genomic DNA, SPRI beads are added to the tube containing the DNA sample and maintained throughout the successive library preparation steps including end-repair, A-tailing, and adaptor ligation.
To precipitate and thus concentrate the protein, a twofold volume of cold acetone was added to each sample and maintained at -20°C for 16 hours, followed by centrifugation at 10,000 g at 4°C for 30 minutes.
After the man had completely recovered 3 months later, another fecal sample was collected (hereafter referred to as the recovery DNA sample) and maintained at –80°C until DNA extraction.
Axenic culture of the green unicellular microalga Chlorella sp., originally isolated from a soil sample and maintained in the Phycology Laboratory at the Centre for Environmental Risk Assessment and Remediation of the Environment (CERAR), University of South Australia, was used in this study.
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The ability to mix aqueous liquids on microsecond time scales, while consuming minimal amounts of sample and maintaining UV-visible optical access to the mixing region, is highly desirable for a range of biophysical studies of fast protein and nucleic acid interactions and folding.
After 2,5 h at 16°C, 5U of T4 DNA polymerase (New England Biolabs #M0203S) was added to the sample and maintaining at 16°C during 40 min.
As shown in Fig. 6a, with an appropriate silver contents of 5 wt.%, the silver-coated Li4Ti5O12 delivered the highest capacity of 186.34 mAh g−1 at 0.5C, which is higher than that of other samples, and maintained 92.69% of its initial capacity at 5C after 100 cycles.
Thus, with an appropriate silver content of 5 wt.%, the Li4Ti5O12/Ag delivered the highest capacity of 186.34 mAh g−1 at 0.5C, which is higher than that of other samples, and maintained 92.69% of its initial capacity at 5C after 100 cycles.
The expression of arterial, venular and lymphatic markers was detected in freshly FACS-sorted samples and maintained in culture up to 70 days, indicating that all three types of endothelial cells (arterial, venular and lymphatic) are included in our sorted starting population and their phenotype is maintained in culture.
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