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For example, on-column sample derivatization will be feasible by simultaneously opening opposing valves containing sample and label.
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Diluted samples and label were incubated at room temperature for 3h before gamma counter reading.
Place the sample in a sample container, and label it appropriately.
Initial matrices are computed by maximizing the centered-kernel alignment (CKA) metric, measuring the affinity between projected samples and labels.
First, in vitro labeling is troublesome due to the necessity of blood sampling and labeling procedure, the requirement for a clean space, and the radiation exposure for operators.
The bottles were rinsed three times and taps were allowed to run for at least 5 min before collection of samples and labelled accordingly.
Sampling and labeling mechanisms are described in Table 1.
An internal standard was prepared by pooling an equal amount of protein from all 6 samples and labeling these with the Cy2-dye as described above.
If patients have both, then they should be separately sampled and labelled as such.
miRCURY LNA Array (v.16.0) (Exiqon, Vedbaek, Denmark) was hybridized with 1 μg RNA samples and labeled using the miRCURY Hy3/Hy5 Power Labeling Kit (Exiqon).
Qualitatively, the peptide overlap between replicate analyses of the same sample and reverse labeled samples were found to be within 8%.
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