Sentence examples for sample and input from inspiring English sources

Purified sample and input DNA was eluted with 50 µl EB buffer.

The sample and input tubes were processed to reverse the crosslinking and DNA was precipitated in ethanol with glycogen as carrier.

The DNA from both sample and input was amplified by semi-quantitative PCR (30 cycles) using the primers specific for the CCL11 promoter (–136 to +61) encompassing both STAT and NF-κB binding sites.

Sample chromatin was incubated with proteinase K for 1 h at 45°C and ethanol-precipitated, then sample and input chromatin were diluted five-fold in PB buffer and purified with QiaQuick columns according to the manufacturer's protocol (Qiagen).

After peak identification, we calculated a p-value for each peak between sample and input, and significant peaks were used for further analysis.

A triangle-based distribution with a median length of 150 bp and 99.0% confidence interval for peak pairs, which were unequal between sample and input, were used.

The amplification efficiency (E) of each primer set was determined during assay development by plotting the cycle thresholds (Ct) from serial dilutions of a suitable cDNA sample and inputting the resulting slope in the equation, E = 10 -1/slope).

(1) Receive signal samples and input the data to the registers and the buffers.

JDR provided control samples and input on the manuscript.

The enrichment of fragmented genomic DNA in the FAIRE samples and input material was analyzed by quantitative PCR.

Chromatin precipitated DNA was resuspended in a final volume of 40 ul of water (both for IP samples and input samples) and 1/10th of each was used for the PCR amplification.