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This image specific thresholding allows corrections for sample and image specific properties, such as background intensity and illumination.
For astrocyte image analysis, three images were captured from each sample and image analysis was performed as previously described.
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A color and a partial transparency are computed for each data sample, and images are formed by blending together contributions made by samples projecting to the same pixel on the picture plane.
The Monte Carlo integration algorithms dominate running time and the relative time spent on sample generation and image sample filtering becomes less important.
Other examples include parameter estimation, stratified sampling and image processing.
DEM results were validated using experimental data obtained from both sampling and image techniques.
Figure 1 Diagram of nanoporous Bi samples and image of the narrow metal strips.
As we mentioned in Section 3.1, the resampling process is composed of main two functions: image sampling and image reconstruction.
The microtome is housed in a freezer unit that maintains the sample at –80°C during sample slicing and image acquisition.
T.M. participated in sample preparation and image acquisition of the stack from the adult OB.
Technical details of sample preparation and image acquisition are discussed in the Methods section.
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