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However, the results in these studies are also sensitive to assumptions regarding the estimation sample and functional form for BMI.
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Therefore, future studies which should include greater taxon sampling and functional experiments should yield more insight into our conclusions.
More in-depth sampling and functional analyses of these genes in the future may reveal more molecular details concerning the adaptation of the Tibetan population to high-altitudes.
However, any speculation for a putative role of this variant or haplogroup Y in Chinese LHON subjects with m.14484T>C should be further tested with more samples and functional assay.
DJS and CS were responsible for earthworm exposure, sampling and functional assays.
Gene expression data originated from Ado-treated and -untreated EPCs samples, and functional similarity was estimated with Gene Ontology (GO -based similarity information.
This may be taken as evidence that VHP is an important regulator of cellular adhesion in glial cells (Fig. 2) and urges for studies involving additional samples and functional read-outs.
To explore this possibility, expression data from this cohort were analysed for associations between expression of EMMPRIN and S100A4 in primary tumour samples, and functional studies were carried out in two colorectal cancer cell lines.
However, it is necessary that additional studies with more detailed data on environmental exposure and survival data of more samples and functional characterizations are needed to confirm our results, particularly in PCa.
Clearly, more detailed analyses of the amplified genes (amongst others in a large cohort of uniformly treated primary tumour samples) and functional studies are required to establish a possible role of one of the new genes in tumourigenesis.
Further taxonomic sampling, and functional studies in Tribolium, will be needed to determine whether the PGC specification mechanisms in these two species may be the result of common ancestry (Fig. 7).
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