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Fluid is injected from the top of the sample and flow through the perforation.
At least 10,000 cells per sample were analysed per sample and flow cytometry data were analysed using FlowJo software.
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Sample collection, sample processing and flow cytometry data collection and analysis were conducted as described in a previous publication.
Attenuation (net balance between removal and release from and to the water column) was estimated in both engineered and natural systems using a comparable model-based approach by considering different uncertainty sources (e.g. chemical analysis, sampling, and flow measurements).
To combat this, some researchers have turned to network flow applications (such as NetFlow, sFlow, OpenFlow and IPFIX) to cut down on data overheads with filtering, sampling, and flow aggregation techniques [55].
We are grateful to the personnel at the Flow Cytometry Unit at Karolinska University Hospital Huddinge for the help with collection of patients' samples and flow cytometry.
KN assisted with samples and flow cytometry.
M.C., J.L. and S.C. coordinated sampling and flow cytometric estimations.
Sampling and flow cytometry were performed as described above.
The optimized conditions for sample pH and flow rate sampling were 6.6 and 7.1 ml min−1, respectively, to obtain the maximum Zn II) preconcentration and determination in the biological samples studied.
The sample and sheath flow rates in the flow tube were 0.3 and 0.6 L min 1, respectively.
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