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The other half was collected immediately for the –Gal sample and fixed for chromatin immunoprecipitation (ChIP; see 'Materials and methods').
For FISH preps, the two fixative components were preheated to 39°, combined immediately before application to the sample, and fixed on a stationary heating block at 39°.
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We need to study Regnerus' sample and fix the mistakes we made 20 or 40 years ago.
Consequently, the dataset does not include bands with fixed presence in one batch of samples and fixed absence in the other batch.
Samples of the liver and the proximal and distal intestine were sampled and fixed in 4% buffered (pH 7.2) formalin.
Segments of diseased or undiseased regions were sampled and fixed in 4% formaldehyde solution.
The microscope slide was air-dried immediately after sampling and fixed in ethanol later on the same day.
The femur specimens were sampled and fixed in 10%% buffered neutral formalin solution until examination.
Selected tissues were sampled and fixed in 10% neutral buffered formalin for histopathologic examination.
Infected leaves were sampled and fixed for 1 h in 2% paraformaldehyde, 2,5% gluteraldehyde.
The granule samples were freshly sampled and fixed by 2% (v/ v) glutaraldehyde for 2 h before ESEM observation.
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