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The interface design enables users to navigate from the peptide view to the sample and experiment levels, tracking all manipulations while updating the aggregate graphs in real time.
Experiment metadata, corresponding to sample and experiment informations were stored in a mySQL relational database.
SeriesMatrix are summary text files related to a GEO series Experiment (GSE) that include sample and experiment metadata together with a tab-delimited matrix that corresponds to normalized expression data.
Triplicates were set up for each sample and experiment was repeated at least twice.
There are projects under way to explicitly capture sample and experiment descriptions in unified ways.
Initial testing had shown that even the more sensitive Gene Array 350 Kit (see below) ideally requires 5 μg of total RNA per sample and experiment.
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PCR was done in triplicate for each sample and experiments were repeated at least three times.
Twenty thousand cells were analyzed per sample and experiments were reproduced independently at least two times.
A minimum of 50,000 events were detected for each sample, and experiments were performed in triplicate.
At least 10 000 cells were analysed in each sample and experiments were performed in triplicate.
Over 20 locations were examined on each sample, and experiments were run in triplicate for each dilution.
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