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Non-irradiated sample and controls incubated for 48 h (a,e,i, andm, respectively).
Sample and controls after irradiation for 30 min (c,g,k, ando, respectively) and incubated for 48 h at room temperature (d,h,l, andp, respectively).
After incubation of cells with extracts of the test sample and controls at 37 °C °C for 24 to 26 h, the culture was examined microscopically for cellular response.
Bars represent 200 nm. Figure 3shows TEM micrographs of the sample Aβ PIAA/AuNP-CLPFFD complex and controls before and after irradiation for 30 min and further incubation (right), and non-irradiated and incubated sample and controls (left).
Figure 2 Intensity of thioflavine T fluorescence signal of Aβ PIAA/AuNP-CLPFFD sample (10 μM Aβ PIAA:0.2 nM AuNP-CLPFFD) and controls Aβ PIAA (10 μM), Aβ PIAA + CLPFFD, Aβ PIAA + bare AuNP (Aβ 10 μM: 0.2 nM AuNP), irradiated for 10 and 30 min and then incubated for 48 h Figure 3 TEM micrographs of Aβ PIAA/AuNP-CLPFFD sample and controls (starting conditions:b,f,j, andn, respectively).
Each honey sample and controls were tested in triplicate by adding 140 μl to each well.
Similar(48)
In Formula (3), A sample and A control represented the absorbances of sample and control wells, respectively.
Limitations: Shortage of the sample and control difficulties of the placebo effect.
Negative control mean values were subtracted from the sample and control group mean values.
The mean blank reading was subtracted from each sample and control reading.
Sample and control wells were seeded and counted in triplicate.
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