Exact(8)
Viable cells were analysed by the addition of propidium iodide (PI; 10 µg/ml) to each sample and cells were analysed on a FACS Calibur flow cytometer.
Data was acquired at 10,000 events per sample and cells sorted with a BD Aria I and later Aria II, both using Diva software.
Six images were taken from each sample and cells were randomly selected from each image for quantification using Image-J.
At least 5,000 events were acquired for each sample and cells positive for PI were gated out.
2 µg purified plasmid-DNA (GFP-α-actinin for myocytes and GFP-VASP for myofibroblasts) was added to the sample and cells were transfected according to the manufacturer's instructions (program G09).
Subsequently, 0.3 g acid-washed glass beads (425-600 μm, Sigma-Aldrich) were added into each sample and cells were lysed using FastPrep-24 (MP Biomedicals, Solon, OH, USA).
Similar(52)
Tissue sample and cell laser microdissection.
Cell number was estimated from a cohort sample, and cell protein content was estimated from the cell pellet.
At least 2 × 10 cells were used for each sample, and Cell Quest software was used for data analysis.
c, Venn diagram showing m6A peak overlap between patient tumour samples and cells (H1299 and A549).
Tissue samples and cells were fixed and embedded in paraffin.
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