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Our results, like those of Cawley et al. ([2009]), can only be viewed as associations since we are unable to instrument for BMI and small sample and cell sizes make individual fixed-effects models infeasible.
In matched FFPE sample and cell lysate studies, there is good qualitative agreement between the VeraTag assay and data obtained from traditional Western blotting or co-immunoprecipitation experiments.
Flow cytometric analysis was carried out on at least 10,000 cells from each sample, and cell cycle data were analyzed using a FACS Calibur flow cytometer (BD BioSciences, San Jose, CA) with an excitation wavelength of 488 nm and an emission wavelength of 530 nm.
Tissue sample and cell laser microdissection.
At least 2 × 10 cells were used for each sample, and Cell Quest software was used for data analysis.
Cell number was estimated from a cohort sample, and cell protein content was estimated from the cell pellet.
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Viable cells were analysed by the addition of propidium iodide (PI; 10 µg/ml) to each sample and cells were analysed on a FACS Calibur flow cytometer.
Data was acquired at 10,000 events per sample and cells sorted with a BD Aria I and later Aria II, both using Diva software.
At least 5,000 events were acquired for each sample and cells positive for PI were gated out.
Six images were taken from each sample and cells were randomly selected from each image for quantification using Image-J.
The San Diego Zoo has its parallel Frozen Zoo, an archive of thousands of DNA samples and cell lines from a host of species.
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