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The following day, distilled water and chloroform (1 1 v/v) were introduced into the sample and allowed to mix for 5 6 h.
For sample preparations, 5 ml of aniline blue solution, 0.001 % (w/v) in 50 mM phosphate buffer pH 7.0 was mixed with 50 mg of sample and allowed to stand at room temperature for 15 min.
We recruited [a couple thousand] guys, who gave us a sperm sample and allowed us to investigate aspects of their lives..
The beads were then pelleted and added to protein sample and allowed to rotate overnight at 4°C.
Anhydrous isobutanolic-3 M HCl (200 µl) was added to the sample and allowed to react at room temperature for 50 min to form the isobutyl ester derivative.
The iTRAQ reagents (Applied Biosystems) were reconstituted in 70% ethanol, transferred to the respective sample and allowed to incubate for 1 h.
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Participants will donate a saliva sample and allow the scientists access to their health records.
APT analysis gives a chemical map of the sample and allows us to measure the composition of each phase.
The methods require only 200 μL of sample and allow a high throughput due to short analytical run (2 min).
Therefore, K pyr can be derived experimentally for a given pyrite sample and allows for the modeling of Phe and Tyr concentrations through time.
The designed system can be trained for a specific sample and allows for unsupervised but efficient data collection imitating the working mode of an experienced microscopist.
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