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The real sample analysis was performed to determine CXB in pharmaceutical and human serum samples.
Sample analysis was performed through a probe having eight 200-µm fiber channels forming a linear array.
Sample analysis was performed in the selective reaction monitoring mode (SRM).
Sample analysis was performed in the selected reaction monitoring mode (SRM).
Sample analysis was performed with an Apogee A40 mini flow cytometer (equipped with a 50 mW 488 nm solid state laser (Coherent).
After preparation of sperm sample, analysis was performed using the CASA system (Version: 10, HTM-IVOS CASA System, Hamilton Thorne Research, Inc., Beverly, MA, USA).
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All the experiment and sample analysis were performed in triplicate at room temperature of 25 ± 1 °C.
To minimize contamination, DNA preparation with setup, PCR, and sample analysis were performed in three separate rooms.
Cytofluorimetric acquisitions and sample analysis were performed with a Partec PAS flow cytometer (Partec, Münster, Germany) and FlowJo 8.6.3 software (TreeStar, Inc., Ashland, OR, USA), respectively.
IHR Core Capacity 8 requires laboratory services for every phase of real-time event management (i.e., detection, investigation, and response), with sample analysis being performed either domestically or through collaboration centers (2).
The mean assay LLOQ was used where samples analysis was performed in multiple technical occasions.
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