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The sample amount was ~0.05 g.
8.33 : conversion factor according to sample amount and KOH concentration.
The detection limit was 0.60 μg g−1 for a sample amount of 30 mg.
The three variables studied were leaching time, concentration of sulfuric acid and sample amount.
The statistically significant variables were sample amount, rinsing time, extractant volume, and acid silica amount.
The most influent factors were sample amount, rinsing time, extractant volume and acid silica gel amount.
Moreover, this methodological advance matches the need of bioscience to downscale the sample amount required for screening devices.
The results showed that there was obvious signal intensity dependence, in particular when sample amount (peak area) was smaller.
However, 4 g of sample amount was selected as optimal value with the aim to improve the specifications of method.
Table 2 Recovery of FA and vanillin Sample Amount in sample (ng) Std added (ng) Total conc.
The bulky nature of nitrogen adsorption measurement (dry state and large required sample amount) is inconvenient when nanoparticles are considered.
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