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In the studies conducted in this field of research, the experimental procedure has involved alternating steps of imaging and ex-situ core sample alteration.
These absorption bands are, however, weak and unclear, partly because it may be related to the sample alteration caused by NanoSIMS analysis (Uesugi et al. 2014).
Indeed, recent experimental investigations show that bulk materials can stand long time periods of electron irradiation before any beam-induced sample alteration is recognized [6].
This could be associated with low viral elimination, sample alteration during shipping or presence of inhibitory factors in urine, since no purification was used.
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Concerns regarding beam-induced sample alterations arise if it is attempted to understand structure and chemical composition of materials at atomic resolution with single atom sensitivity.
This contribution addresses electron beam-induced sample alterations in atomic resolution electron microscopy using acceleration voltages between 80 and 300 kV and beam currents that range from atto-Amperes/Å2 atto-Amperes/Å2/Å2.
Consequently, a variety of strategies are now deployed [3, 5, 15, 17, 18] that aim at overcoming the various aspects of electron beam-induced sample alterations at the single atom level in atomic resolution electron microscopy.
As a consequence, unintentional sample alterations are often obscured by a lack of sensitivity, the choice of forgiving detection modes, the absence of time resolution, and other factors [16], such that they are easily overlooked and may trigger misleading conclusions.
It was recently demonstrated that electron beam-induced sample alterations can be effectively retarded by recording images with low dose rates and accumulating the needed total electron dose by acquiring image series that contain hundreds of frames [26].
Other buffers may not be equally suited to Coulter counter measurements and dilution in saline or other conductive solutions will raise the question of potential sample alterations caused by changes in solution conditions and the sample preparation process.
This section discussed the arbitrary sampling alteration, a very important point when the frequency varies continuously.
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