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The vacuum GIWAXS sample chamber is equipped with a 5-axis motorized stage for fine sample alignment.
Olympus 10× UPlanFL N 0.3 NA air objective was used for sample alignment and large field of view fluorescence imaging.
For calculating the tensile stiffness, the slope of the initial linear region of the stress strain curve following sample alignment was determined.
Firstly, a motorised x,y,z sample mount capable of holding an APD cryostat has been developed; this device is useful not only for initial sample alignment, but also in mapping a sample for the location of single crystallites.
Analysis questions: Sample alignment: 1.
A tool recently developed (Riddell et al. 2012) will allow more detailed examination of within-tree patterns and eliminate problems associated with sample alignment (with respect to stem axis).
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We calculated the coverage of the individual sample alignments versus the Y.
Quality score recalibration based on sample alignments to a reference genome has been identified as one solution to this problem [ 43].
Alignment to individualized genomes presents a challenge for comparison and visualization of sample alignments; however, new tools make conversion back to reference genome coordinates straightforward (Huang et al. 2013).
Sampling Alignment Structure Under a Bayesian Translation Model.
Due to the potential for slight variances in indel placement between MAVID and BWA/GATK alignments, indel events identified in read pool mapping were considered called correctly if they occurred within three base pairs of an indel event identified in the reference-sample alignment.
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