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The best retention properties for methyl-mercury, inorganic mercury and ethyl-mercury species from seawater were obtained when loading 200 mL of sample adjusted to pH 8.0 and at a flow rate of 2.0 mL min−1 on a column-packed with 200 mg of the material.
1 μL of the extracted sample adjusted to 50 μL/well with cholesterol assay buffer was used per assay.
The following day, samples will be thawed and the pH of each sample adjusted to 6.5 using 4 N KOH.
The load material consisted of the dialysed sample adjusted to 3.5 M NaCl, acidified to pH 5.5 using acetic acid diluted 1 20 and filtered 0.22 μm.
Cartridges were washed with 5 × 2 mL of water adjusted to pH 7. One-half liter of the water sample adjusted to pH 7 was glass-fiber filtered (< 1 μm) and spiked with bisphenol F as surrogate standard.
Genomic DNA was extracted using a modified Tanksley extraction method (Fulton et al. 1995) and sent for SNP analysis (10 µg DNA per sample, adjusted to 100 ng/µl).
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Slow speed rotation drum for ~30 samples adjusted to 45° angle.
ζ -Potential of samples, adjusted to different pHs with 1.0 M nitric acid or 1.0 M KOH using an autotitrator model BI-ZTU (Brookhaven Instruments Co., Holtsville, New York, USA), were determined.
Samples, adjusted to 40 mM Tris (pH 6.8), 7% glycerol, 1.5% SDS and 400 mM 2-mercaptoethanol, were then fractionated on 12% SDS-PAGE gel.
Protein content was measured by the BCA protein assay (Pierce) and samples adjusted to equivalent protein concentrations with sterile water.
All PCRs were performed using cDNA samples adjusted to equal glyceraldehyde-3-phosphate dehydrogenase (G3PDH) inputs under conditions that permit exponential accumulation of PCR products.
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