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We agree that using the Jurkat cell line as a model system for studying regulation of the cell cycle introduces important limitations due to its origins as a leukemia sample adapted for continuous growth in culture.
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The method can detect 48 SNPs in one single well for each patient sample, adapted here to a 96-well plate format covering more than 400 SNPs for cancer chemotherapy.
Each participant was provided with a saliva sample collection kit (Sarstedt Corp., Montreal, Canada) with stepwise instruction for collecting saliva samples adapted from sample collection procedures described in the literature (King et al. 2000).
A sample arm adapted for retinal imaging and a conventional reference arm were constructed.
Here we present a coaxial transmission line setup originally designed for soil samples, now adapted for measuring ice samples between 10 MHz and 1.5 GHz.
Dilutions of plasma samples were adapted for each assay.
In addition, since Jurkat cells are derived from a human T cell leukemia sample that was adapted for continuous growth in culture, its cell cycle regulatory machinery may not be representative of primary T cells.
Open image in new window Fig. 4 Development of maximum quantum yield over time; samples were dark adapted for 5 min.
Although diatoms were detected on day 1 and after day 5, Y decreased below 0.7 and the maximum NPQ did only reach a maximum of around 1.5 after day 7. Open image in new window Fig. 6 Development of maximum quantum yield and non-photochemical quenching over time; samples were dark adapted for 5 min.
Samples were dark adapted for three minutes and exposed to brief saturating light flashes (0.7 µs flash, 1.5 µs darkness for 600 cycles) to measure the fluorescence from fully oxidized (Fo) to fully reduced (Fm) PSII reaction centers.
All samples were dark adapted for two minutes before measurement.
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