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Researchers assessing the CAR thus need to take important steps to maximize and monitor saliva sampling accuracy as well as consider a range of other relevant methodological factors.
All samples underwent immediate testing in duplicate to provide inter-sample accuracy as well as ensure precision of the values obtained, and all samples were stored under refrigeration, not frozen.
In previous work we demonstrated that gene component analysis could discriminate estrogen receptor (ER) positive and negative breast cancers and gene component classifiers could be projected into independent samples with high predictive accuracy, as well as an integrated step of automatic gene selection [ 8].
Besides the parameters of SI as discussed in this study, we can investigate the influence of the point sampling granularity on the identification accuracy as well in the future.
Durango apatites (McDowell et al. 2005) were also run as "unknowns" with each batch of samples as a check for sample accuracy.
Fish Canyon Tuff zircons (Gleadow et al. 2015) were also run as "unknowns" with each batch of samples to check sample accuracy.
Raman spectra were collected in different areas of each sample for accuracy, as non-uniform spots on the surface were visible under the light microscope.
ALCHEMY has been shown to obtain >99% accuracy with as few as six samples, with larger numbers of samples continuing to improve call rates.
Initial experimentation was carried out to simplify the weighing out of samples, whilst conserving accuracy, as this is a time consuming part of the screening process.
Two test runs were carried out for each lignite sample with accuracy and reproducibility as per as per DIN 51730 (1998–4).
For most STR-based methods, quality control (QC) relies on statistical procedures, such as running samples with a known accuracy as controls and assuming that unknown samples will perform the same way.
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