Carbonaceous chondrites are valuable meteorites that only comprise about 5%% of observed falls; therefore, sample abundance is a common concern for meteoritic analyses.
Nevertheless, either carbon, nitrogen, or hydrogen CSIA offers valuable data, the targeted isotope is chosen depending on factors such as the compound being analyzed, sample abundance, and possible sources of contamination.
Available from any single sample, abundance estimates are usually adequately modelled as a function of landscape descriptors, and as such offer considerable advantages over the direct modelling of species richness.
These 20 samples were chosen because of sample abundance.
Binner has the optional parameters minimum sequence identity and minimum sample abundance, but these were not applied in our analysis.
A third sample was analyzed when results of the duplicates differed by more than 10% (blood) or when sample abundance permitted (hair).
Nevertheless, using a similar input of a particular tissue type, putative selective expression of a given protein would be more likely to derive from extraction method specificities rather than derive from sample to sample abundance.
In order to separate individual Sulfobacillus bins, a discrete bin containing putative members of the order Clostridiales, was isolated and further binned using log-normalized sample abundance patterns from 18 different samples, this time including fragments down to 1500 bp.
Some of the 20 proteins significantly altered in db/db vs dbM mice, but not in wild-type vs db/db mice, could have resulted from variations in sample abundance (Fig. 6a, b).
(Additional file 2: Table S1) It is also important to note that FPKM units should not be used to calculate the enrichment fraction (Additional file 3: Figure S2), as the FPKM derivation [ 6] includes a term coupling sample abundance to spike abundance.
