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All animals in that prior study received the same titrated hemodynamic and ventilatory support as the animals in the present study received.
A standard curve for virus quantification was established with RNA extracted from dilutions of the same titrated stock of each virus used to inoculate the chickens and was run in triplicate.
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The same PEEP level titrated at this time was used in the following steps of the study.
Antibody titres were initially determined by a standard ELISA on plates coated with PfAMA1 from FVO, HB3, 3D7 and CAMP parasite strains respectively, and titres were comparable to those obtained when the same antibodies were titrated on the immunising DiCo antigens (Figure 1).
However, we attempted to control for instrumental differences by including the same positive control titrated in each assay in order to provide data to estimate the amplification efficiency for each run.
The Fyn SH3 domain peptide was dissolved in the same buffer and titrated into the xα2-peptide solution to observe the chemical shift changes of the xα2-peptide in 2D N-HSQC spectra at different molar ratios (1 0, 1 0.1, 1 0.2, 1 0.4, 1 0.5, 1 0.6, 1 0.7, 1 0.8, 1 1.0, 1 1.2, 1 1.2).
If glargine had been titrated in the same manner to twice-daily dosing based on the same titration as detemir, glargine would have been dosed twice daily as often as detemir and possibly would have been higher.
50 mL each of the distillate and a blank were titrated under same conditions using 0.1 M H2SO4.
DNAseI activity was previously titrated, and the same batch of enzyme and thermocycler were used with the same settings across all experiments to obtain reproducible fragmentation.
Circles correspond to the solute dissolved in the phosphate 0.1 M and pH 7.4 media and titrated into the same buffer while squares represent the solute dissolved in pure water and titrated into the same solvent.
A separate experiment was then carried out with the same substrates, but titrating them in the reverse order.
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