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This study fills this gap by using the same sample for its SEM models.
As such, by using the same sample for its weekday and weekend models, this study can directly compare their analytical results.
Unlike previous studies, it uses the same sample for two models that are specified to test the relationship separately for weekday and weekend travel.
The mean VH/PVH level of each group was calculated from the mean value of three repeated analyses of the same sample, for which the coefficient of variation was calculated.
Multiplexing of such samples to enable analysis of multiple libraries mixed into the same sample for sequencing can also render the process more cost effective and allow for the analysis of more samples.
Using the same sample for more than one consecutive season could lead to biased coverage estimates.
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To keep the estimates comparable, we use the same samples for regressions using different methods.
To optimize cost-effectiveness, it is often wise to make use of the same samples for both chemical and effect-based analyses.
Densitometry was performed on a separate western blot using the same samples for the other genotypes (Figure 5C).
We therefore tested the same samples for keratin 8 cleavage to rule out major differences between our infection conditions and the published ones.
The results of these imaging experiments are summarized in Figs. 4 and 5 (as well as Appendix S1, see e.g., Figures S3 and S4), which also provide conventional fluorescent microscope images of the same samples for comparison purposes.
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