The same quantification for the positive FITC-Annexin V labeling of SGNs was performed.
To a broader extent, one of the most practical questions researchers want to know in advance is: if different gene models are chosen for RNA-Seq data analysis, what is the chance of obtaining the same quantification result for a given gene?
Absolute qPCR allows the precise quantification of the target DNA/cDNA based on a standard curve constructed in the same quantification assay as the question samples.
At the same time, quantification of the cholesterol levels over 24 hours of treatment showed only modest or no changes in cells treated with BN108 or TAIII (Figure S2).
Since a split FISH signal would display the same pattern, quantification of the signal intensity over a minimum of 30 metaphases was performed on this CML patient sample as well as a series of cytogenetically normal individuals [see Additional file 3].
Although the two approaches are conceptually rather different, they address the same issue – the quantification of areal rainfall intensities and their related measurement errors – and a comparison is hence of interest.
We found that densitometric analysis of the CFGE gels after staining with ethidium bromide gave exactly the same results as quantification of the radioactivity in the gels.
Surface distances (mm) between two different time acquisitions within the same animal allowed quantification of the displacement fields describing mandibular growth.
Quantification of SAMe and SAH was performed as described previously (Burren et al., 2006).
The same requirement applies for quantification of phosphorylated full-length TDP-43 without interference from phosphorylated C-terminal fragments.
