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Our B-modeling of diffuse scattering allows a closer connection between haze and both physical and physiological quantities, while yielding the same quantification of haze as the standard method.
Using the same quantification and calculation methods as described in the legend of Figure 2, the copy numbers of two OATP1B3 mRNA isoforms in each sample were separately determined.
Absolute qPCR allows the precise quantification of the target DNA/cDNA based on a standard curve constructed in the same quantification assay as the question samples.
The same quantification was done for DARPP-32-immunoreactive neurons and the percentage of TO-PRO-3-labeled cells that were DARPP-32-positive neurons was calculated.
We confirmed these finding by measuring EB3 movements in several cells and obtaining essentially the same quantification results (data not shown).
The same quantification for the positive FITC-Annexin V labeling of SGNs was performed.
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To test this prediction we did the same quantifications in embryos heterozygous for a Dyrk1a null mutation (Dyrk1a+/− embryos) (Fotaki et al., 2002).
At the same time, quantification of the cholesterol levels over 24 hours of treatment showed only modest or no changes in cells treated with BN108 or TAIII (Figure S2).
We do not foresee major difficulties to the prediction of independent samples using the gene signature, provided the same expression quantification technology is used (Affymetrix GeneChip©).
LINE-1%meth levels observed in VAT of the study subjects were comparable to levels reported in previous epidemiological studies using white blood cells (WBC) and the same methylation quantification method [ 18, 23].
Since a split FISH signal would display the same pattern, quantification of the signal intensity over a minimum of 30 metaphases was performed on this CML patient sample as well as a series of cytogenetically normal individuals [see Additional file 3].
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