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However, when the CB1 agonist ACPA was applied to the same preparations that had been pretreated with U-73122, the EPP amplitude was decreased by an amount (53.8 ± 5.2%) that is indistinguishable from the effects of either ACPA or muscarine in the absence of U-73122 (compare Fig. 7B with Fig. 2).
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This result is not consistent with the functional data from the same preparation that show an upregulation by 1,25- OH 2vitamin 1,25- OH 2vitaminD3Ca2+ fluxes in primary cultures of SCCD [ 7].
In order to determine the genome-wide gene expression, the same cDNA preparations that were used for SM RT-PCR were also employed in microarray hybridization experiments.
Primers specific for their corresponding rat sequences (Additional file 6 Table S6) were used to analyze the same mRNA preparations that had acted as sources for the microarrays.
To confirm the results from microarray experiments, real-time quantitative RT-PCR was carried out using the same RNA preparations that were used in the microarray experiments.
Twenty-five diffexpressedy expressed genes were randomly selected and validated with the same RNA preparations that were used to generate microarray data, and 8 out of the 25 genes were again validated with the new RNA samples from the same ovary tissues by qRT-PCR.
Such activity is absent from the cytosolic fraction of the same preparations showing that the bioactive component, BylA, is secreted.
Historically, it is documented that humans utilize the same herbal preparations that they use to treat their sick animals [ 1].
To this aim the same RNA preparation that had been employed for microarray analysis was used for determination of rRNA synthesis rates by a quantitative primer extension assay [ 10].
Guinea pigs and ferrets each received 50,000 50% tissue culture infectious doses of the same virus preparation that was used in the horses; guinea pigs received the dose by intraperitoneal injection, and ferrets received the dose by the oronasal route.
Red blood cell (RBC) agglutination provides evidence that rHA is in its native trimeric form [ 14], and therefore the response for rHA of B/BR/08 that has measurable HAU was compared to the response measured for the same rHA preparation that had been heated to 95°C for 5 min and no longer agglutinated RBC.
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