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However, for those deletions and insertions for which this extension resulted in the same nucleotide as was present in the wild-type DNA, optimal discrimination was achieved by oligonucleotides extending further into the deletion or insertion.
We searched for sites within 20 bp of the heteroplasmic base substitution site that were the same nucleotide as the mutant base; likewise, we also searched for nearby sites that were the same nucleotide as the progenitor (wild type) site.
The functionally neutral variant, −215G>T, affects the same nucleotide as the abovementioned functional −215G>A variant.
Note that the two edges of an SSR of exactly X repeated units cannot be the same nucleotide as the SSR itself.
If both genomes exhibited the same nucleotide as one another and as one of the two human alleles, we inferred that this nucleotide was the ancestral state.
In each case, one nucleotide was changed to obtain the same nucleotide as in RM11-1a (in which the respective TFBS was not predicted in our scan).
Similar(51)
In our pool of predicted varietal SNPs, only ~3% were of these types that should behave as codominant SNP assays, the rest potentially behaving as dominant SNP assays because the other subgenome homeologs carry the same nucleotide at the SNP position as one of the alleles.
The plasmid pMARCKS-Luc(−736/mt) was generated by site-directed mutagenesis, which changed the same nucleotides as those of mutant 5. Human neuroblastoma SH-SY5Y cells were obtained from the Human Science Research Resources Bank HSRRBB).
The SRV-Vero of JCRB0111 had 97% of the same nucleotides as those of ATCC CCL81 (Genbank_ID: JN134185).
We also found four other non-synonymous SNPs in this gene whose mutant alleles share the same nucleotides as the donor sequences.
In addition to the character set defined by the IUPAC code we used corresponding small letters that represent the same nucleotides as the respective capital letter plus the deletion.
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CEO of Professional Science Editing for Scientists @ prosciediting.com