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Mock-irradiated samples were collected after the same length of incubation.
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The same experiments also revealed marked differences in precipitation depending on the length of incubation allowed following the addition of CaCl2 and Na2HPO4 (for brevity, these will be referred respectively as calcium and phosphate treatments in the text).
Under the same experimental conditions, different routes of administration show different efficacy of disease transmission, in terms of length of incubation period and percentage of infected animals [ 16, 17].
Thus, energetic demands of incubation should constrain incubation-scheduling, that is, the length of incubation bouts, their constancy (the amount of time birds actually incubate within an incubation bout), and their timing.
Again the length of incubation time may be selected simply to parallel that of the primary assay.
The original method involved a variable length of incubation in USP followed by centrifugation.
Dependent on the length of incubation fragments became increasingly smaller (data not shown).
Turbidity continued to increase with the length of incubation (2 months).
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