Samples were washed three times in the same buffer, post-fixed at room temperature in 1% osmium tetroxide in 0.1 M sodium cacodylate buffer, washed three times in the same buffer, dehydrated in an acetone series and embedded in Polybed 812 resin.
The cells were then washed in the same buffer, dehydrated in acetone, and embedded in Epon.
Parasites were washed in the same buffer, dehydrated in acetone and embedded in Epon resin.
Samples were washed with the same buffer, dehydrated with increasing ethanol series, treated with hexamethyldisilazane and air dried.
After washing, they were fixed in osmium tetroxide for 2 h in the same buffer, dehydrated through an acetone series and embedded in Spurr resin by infiltration.
Nuclei were post-fixed for 2 hours in 1% osmium tetroxide in the same buffer, dehydrated with ethanol, and embedded in epon 812 resin [39] (TAAB, England).
The fragments were post-fixed in 1% osmium tetroxide in the same buffer, dehydrated in graded alcohols, embedded in Epon 812, sectioned with an ultramicrotome, and stained with uranyl acetate and lead citrate.
Cells were fixed in a solution of 2.5% glutaraldehyde grade II, 2% formaldehyde and 2.5 mM CaCl2 in 0.1 M sodium cacodylate buffer pH 7.2, post-fixed in 1% osmium tetroxide and 0.8% potassium ferricyanide in the same buffer, dehydrated in an acetone series and embedded in Polybed resin.
Samples were washed twice in the same buffer, dehydrated in acetone and embedded in PolyBed 812.
Specimens were post-fixed in 1% osmium tetroxide in the same buffer, dehydrated with graded series of ethanol and embedded in epon, as previously described [ 21].
