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It should also be noted, that PrP, as well as the studied peptides (with the exception of the signal peptide, MAN28) lost their antimicrobial activity at high salt strength, casting doubt of the potential antimicrobial role of PrP in vivo.
On the other two resins, P34 adsorbed stronger and only a very small amount of P34 could be eluted at this salt strength (lanes 8, 10), together with other contaminants.
As in Figure 1A, ion exchange fractionation of plasma yielded two separate zones of SR-A binding activity, and again the second zone had very weak absorbance at 280 nm and eluted at a high salt strength.
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36 Due to a salt policy enacted in the US and European Union, the strength is expressed in terms of the active moiety (brimonidine 0.33%) rather than the salt-strength equivalent that was used in the clinical trials (brimonidine tartrate 0.5%).
Salinity strength was defined by seawater concentration adjusted by artificial sea salt in this study.
"These yeast tolerate some unusual conditions, such high and low pH, high salt, ionic strength, and human urine," Blenner told MUNCHIES.
Salicornia plant roots were dipped in MS salt half strength medium (SIGMA, Italy) supplemented with 2% NaCl and the prepared bacterial suspension.
Other distinctions between thermophilic and mesophilic proteins might be due to differences in their structural properties, such as different amino acid compositions, loop lengths, number of salt bridges, strength of hydrophobic interaction, number of disulfide bonds, and other features [ 18- 25].
In the presence of high ionic strength salt NaCl, the zeta potential does not alter.
The electrolytic concentration was kept very low (1.5 mM) to render the measurements independent to the salt concentration (ionic strength).
The uncharged backbone of PNA makes PNA/DNA or PNA/RNA hybridization independent to salt concentration, ionic strength, and changes in pH.
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