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Before adding the alkaline extract to the culture to assay mitogenicity, the soluble fraction of the pH 9 extraction was neutralized with Hepes buffer; the salt and detergent in salt extract and detergent extract were removed by dialysing the sample in 1 litre of 20 mM phosphate buffer (pH 7.4) plus 0.15 M NaCl overnight with two changes of dialysis buffer.
Later, molecular biological techniques were used both to clone genes encoding products found in the salt extract (BCSP31 and Cu Zn SOD) and genes encoding proteins of B. abortus that were antigenic (HtrA) or possibly essential (two-component systems) for full virulence of B. abortus.
Figure 2a shows the chromatogram obtained from the preparative HPLC for the oxalate salt extract.
A salt extract of microsomal membranes contained the major portion of enzyme activity, indicating that this acyltransferase is not an integral membrane protein.
BALB/c mice were immunized intraperitoneally with 25 µl of a 1 mg/ml solution of a high salt extract of F. tularensis subsp. tularensis SCHU S4 prepared according to the method of Hood [16].
In order to determine if F. tularensis SCHU S4 produced a capsular antigen and to develop a probe for it, we elected to make monoclonal antibodies (MAb) to a high salt extract of F. tularensis subsp. tularensis SCHU S4 prepared according to the method of Hood [16].
Similar(41)
The supernatant was collected as the salt extracted fraction and the pellet resuspended in 50 μl Salt Extraction Buffer to generate the chromatin fraction.
These values were compared to the amounts of salt extracted by the respective poultices.
Cities on overland trade routes grew rich by levying duties, and towns like Liverpool flourished on the export of salt extracted from the salt mines of Cheshire.
The salt extracted from this operation may have had a direct correlation to the rapid growth of this society's population soon after its initial production began.
To determine whether the nuclear Dnmt2 fraction is associated with the nuclear matrix we salt extracted purified nuclei.
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