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Several DNA applications of developed microparticles were described, among others RNA and DNA degradation and Salmonella cell magnetic separation by RNase A and DNase I and anti-Salmonella or proteinase K immobilized on developed magnetic carriers.
The probability of detection was 100% when a Salmonella cell suspension containing 101 CFU/ml was used as a template in the LAMP assay.
The chip is able to isolate DNA with high recovery efficiency (96± 11%) in an extremely large dynamic range of prepurified Salmonella DNA as well as from Salmonella cell lysates that correspond to a range of 5 to 1.9 × 108 cells (0.263 fg to 2 × 500 ng).
There was a highly significant reduction in Salmonella cell viability [19].
Different Salmonella cell concentrations (10-109 CFU/mL) were immobilized on to the MAb-DW electrode and the CA measurements were conducted to obtain the detection range at the electrode.
After washing IPEC-J2 cells 3 times with PBS to remove non-adherent Salmonella, cell culture plates were analyzed by the Aklides apparatus (GA Generic Assays GmbH, Germany).
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All labels released ClO2 at concentrations capable of complete inactivation of Salmonella cells on TSA plates.
Salmonella cells were then successfully identified using cultural and polymerase chain reaction (PCR) methods after their immunomagnetic separation.
Mathematical models were then developed to quantify the combined effect of these parameters on heat resistance of starved Salmonella cells.
The experts urged salad growers to maintain high food safety standards given the potential for a few salmonella cells in a bag at the time of purchase to increase to many thousands by the use-by date.
We apply and validate the model for the case where analytes are grouped on Salmonella cells, and ligands are anti-Salmonella antibodies.
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