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There was no difference caused by incubation atmosphere but the tests in PBS gave a positive reaction for α-fucosidase in both atmospheres that was not recorded in the isotonic saline tests.
The calculation was based on previous studies about the prevalence of positive saline tests among patients with chronic cough and the responsiveness of these patients to ICS [ 7, 13].
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For the latter condition, sexual conditioning persisted from cocaine training to the saline test.
In the cough patients, the first EBC was collected after the spirometry, before the saline test, and the second two minutes after the saline test.
The saline test did not affect the EBC 8-isoprostane concentrations: There were 100 pairs of EBC samples collected before and after the saline test.
Neither the response magnitude nor the speed was predicted by the saline test.
Histamine challenge and the saline test were performed on separate days.
Those responding to the first saline test repeated it and the nitric oxide measurement during the subsequent visits.
The healthy subjects did not perform the spirometry and the saline test but only underwent the EBC sample collection.
Before therapy, spirometry, ambulatory peak flow monitoring, nitric oxide measurement, histamine airway challenge, and saline test were performed.
The challenge was stopped if 15 or more cumulative coughs were recorded (= a positive saline test result).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com