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Our previous paper [ 7] clarified that even the addition of only 5% human serum to the bacterial suspensions allowed bacterial survival in comparison to samples in saline tested with the same gaseous ozone concentration and time exposure.
For the latter condition, sexual conditioning persisted from cocaine training to the saline test.
In the cough patients, the first EBC was collected after the spirometry, before the saline test, and the second two minutes after the saline test.
The saline test did not affect the EBC 8-isoprostane concentrations: There were 100 pairs of EBC samples collected before and after the saline test.
Neither the response magnitude nor the speed was predicted by the saline test.
Histamine challenge and the saline test were performed on separate days.
Those responding to the first saline test repeated it and the nitric oxide measurement during the subsequent visits.
The healthy subjects did not perform the spirometry and the saline test but only underwent the EBC sample collection.
Before therapy, spirometry, ambulatory peak flow monitoring, nitric oxide measurement, histamine airway challenge, and saline test were performed.
The challenge was stopped if 15 or more cumulative coughs were recorded (= a positive saline test result).
The saline test predicted neither the magnitude nor the speed of the budesonide response (Tables 2 and 3).
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Justyna Jupowicz-Kozak
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