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Saline samples were analyzed at 37°C and corrected for temperature.
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With the analysis of selected duplicates and of damaged duplicates of selected components, a total of 48 samples were analyzed by saline extraction.
To measure LC3 flux in vivo, mice were treated with an i.p. injection of either saline or leupeptin (20 mg kg−1 b.w). 2 h before tissue harvesting and samples were analyzed by immunoblot for LC3.
After washing the cells twice in Dulbecco's phosphate buffered saline supplemented with 2% FCS and fixation in 1% paraformaldehyde (Ph = 7.4), samples were analyzed by flow cytometry using FACScalibur (Becton Dickinson, San Jose, CA) instrument.
Triplicate samples were analyzed.
The samples were analyzed before and after immersion in the above mentioned solution as well as in 0.01 M NaOH solution (pH=12), saline phosphate buffer at pH 7.4 (SPB) and esterase/SPB.
Calibration verification samples were analyzed prior to analyzing samples.
Samples were analyzed immediately.
Samples were analyzed in quadruplicate.
All samples were analyzed anonymously.
All samples were analyzed twice.
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