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In contrast to the data for the blood-perfused kidney, the DWS autocorrelation functions for saline perfusion depend only very weakly on flow rate.
The perfusate exiting the right atrium was clear at the completion of saline perfusion.
Finally, the micro-perfused electrodes take advantage of enhanced RF delivery with the help of saline perfusion.
These animals received basal saline perfusion but were not treated with any further hemodynamic support after LPS injection.
Saline perfusion also increased E2 levels in caudal telencephalon.
Where possible, saline perfusion may be best avoided altogether.
The observed changes in brain steroid levels after saline perfusion show that saline perfusion has its own direct effects on the brain.
Saline perfusion decreased DHEA concentrations by ∼25% in caudal telencephalon and by ∼50% in diencephalon, while saline perfusion increased E2 concentrations by ∼100% in caudal telencephalon.
Next, we examined the effects of saline perfusion on brain steroid concentrations.
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For mice given control injections of saline, the perfusion results in complete flushing of the blood and EBD from the liver.
BW: body weight; L-NAME: Nω-nitro L-arginine-methyl ester; M3R: muscarinic 3 receptor; NO: nitric oxide; NOD: nonobese diabetic; NPY: neuropeptide Y; PBS: phosphate-buffered saline; PU: perfusion units; SS: Sjögren's syndrome; VIP: vasoactive intestinal peptide.
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