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a Gradient plate technique; drug gradients prepared for acetaminophen (Ace), acetyl salicylic acid (Asa), benzoate (Ben), diclofenac (Dc), ibuprophen (Ibu), and sodium salicylate (Sal); concentration gradient provided in parentheses.
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To investigate the effect of oral contraceptive (OC) use on salivary testosterone (sal-T) concentrations and performance-related statistics in international field hockey matches.
The liposomes (GTS-lip) were prepared using film hydration method combined with probe sonication to encapsulate two hydrophobic components (TSN and GA), and using pH gradient method to load hydrophilic component Sal B. The concentration of encapsulated drugs was measured by a reversed phase high performance liquid chromatography (RP-HPLC) method.
The prototype compound (Lys-Sal)4 carboxamide weakly associates in aqueous solution at physiological salt concentration in a monomer-dimer-hexamer equilibrium.
As shown in Figure 1(a), Akt activation was increased by Sal, while increasing concentrations of Sal induced a reduction in total Akt protein levels.
Our results show that β-tan and Sal A, at concentrations that did not inhibit JB6P + cell proliferation, were effective in reducing TPA-induced proliferation and inhibiting TPA-induced colony formation.
Interestingly, we found that high concentration of Sal increased pAkt, pGSK3 β, pTSC2, and p4EBP1.
In some experiments, various concentrations of SAL were added into the growth media as indicated in the following experiments.
However, compared to those grown in other concentrations of SAL, viable KP-M1 cells were much less in 300 μg/mL of SAL.
No significant difference was found between the number of viable KP-M1 cells cultured with or without the presence of various concentrations of SAL.
In conclusion, high concentrations of Sal can be used to reduce total Akt levels or prevent the generation of its activated form, pAkt.
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