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While insertion mutants of AtPV42a were not available in all public resources searched, a SAIL line (CS823876) containing a T-DNA insertion at the last exon of the AtPV42b gene was obtained from Arabidopsis Biological Resource Center and named as atpv42b-1 (Figure 1A).
The SAIL line contain a pCSA110 vector with a 1'2' dual promoter near the left border, the GK line contain a pAC161 vector with a 35S promoter at its right border, and the SALK lines contain a pROK2 vector with a 35S promoter near the left border.
A ProMAS-BAR-35Ster resistance cassette flanked by EcoRI-sites was amplified from genomic DNA of a SAIL line containing a T-DNA derived from pDAP101 into the EcoRI site of the minimal pCAMBIA to generate pCAMBIA-proMAS-BAR-35St (pCMBAR).
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The crew spent the following Sunday and Monday salvaging what they could from the boat — hydraulics, sail lines, fluids, electronics, and hardware — as a coast-guard plane dropped food packages.
It creates a chill and an ambiance, there are lots of interesting noises at night, seabirds, the squealing of the dock lines and docks, the sound of sail lines gently beating on masts.
For a near-term sail performance comparable to that of NASA's Sunjammer sail, the results show transfers that indeed require very little steering effort: the sail acceleration vector can be bounded to a cone around the Sun-sail line with a half-angle of 7.5 deg.
The approximately optimal sailing line and short sailing time are obtained using the AC algorithm.
From a tracking error viewpoint, the switching law determines that the approximately optimal sailing line and the shorter sailing time are obtained using the AC algorithm, and that uncertain nonlinear factors are compensated by the FNN controller.
This V-shaped pattern is formed by two loci of cusps whose angle with the sailing line is 19°28′ in deep water and 54°44′ is the angle between the sailing line and the diverging wave crest line at the cusp of the local line as shown by Ursell [14].
This V-shaped pattern is formed by two loci of cusps whose angle with the sailing line is 19°28′ in deep water and 54°44′ is the angle between the sailing line and the diverging wave crest line at the cusp of the local line.
The two troopships and Port Fairy were sailing line abreast (Duchess of York was the westernmost of the three ships, California was in the centre and Port Fairy at the east of this group), Iroquois was located 3000 yards ahead and the two other escorts were on each flank of the troopships.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com