Sentence examples for safe buffer from inspiring English sources
Turkey could ask for the creation of a safe buffer zone along its Syrian border in the fight against Isis.
Keeping a safe buffer of air between your attack and target is paramount and mistakes can result in some grievous injuries.
After treatment with permeabilisation buffer (Takara Bio) for 2 min on ice, the cells were incubated with TdT enzymes plus labeling safe buffer (Takara Bio) at 37°C.
Subsequently, tissues were incubated with pre-cooled labeling reaction mixture containing labeling safe buffer and TdT at 37°C for 5 30 min after PBS wash.
Sections were incubated with Labeling Safe Buffer containing TdT Enzyme and Biotin-conjugated dUTP at 37 °C for 60 min. Tissue sections were stained with FITC-conjugated extravidin and then counterstained with methyl green.
A total of 50 μL of labeling reaction mixture (5 μL TdT enzyme + 45 μL labeling safe buffer) was applied to slides and incubated in a 37°C humidified chamber for 90 min.
For the one-step RT-PCR protocol, cDNA was generated from total RNA diluted 10-fold in RNA-safe buffer (50 ng/µl carrier RNA, 0.05% Tween 20, 0.05% sodium azide in RNase-free water) [10] by RT-PCR with a SuperScript III one-step RT-PCR system with Platinum Taq high-fidelity polymerase (Invitrogen).
1.5.4 Assemble the Plasmid-safe nuclease reaction (15 μl ligation reaction 3 μl 10× Plasmid-safe buffer 1.2 μl 25 mM ATP 1 μl Plasmid-safe nuclease (Epicentre) 9.8 μl water).
miRNAs generally act as "fail-safe" buffers against gene misexpression in time and/or space, in effect canalizing the transcriptome (Carrington and Ambrose 2003; Stark et al. 2005).
Phospho-safe lysis buffer (Pierce) was supplemented with protease inhibitors (Roche).
