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So here are some of the sad data from the report.
A complete SAD data set from Se-Methionine derived crystal (3.2 Å) has been collected to solve the structure.
The selenomethionine SAD data set of the EBOV NPcore was collected at 2.4 Å using a wavelength corresponding to the Se peak at the SSRF (Shanghai, China) beamline BL19U, and another native data set was collected at 1.8 Å.
Excluding the first selenium of each polypeptide, 6 of 7 selenium atoms in the asymmetric unit were located and refined, and the SAD data phases were calculated and substantially improved by solvent flattening using the PHENIX program (Adams et al., 2002).
The SAD data set for Se-Met Get3 crystals were collected at SER-CAT in APS.
Additional iodide sites were identified using anomalous difference Fourier maps, and all iodide site occupancies were determined by refinement of the SAD data directly within REFMAC [48].
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The initial phasing was carried out using Oasis [29] and Solve/Resolve [30] using the 2.9 Å Se-SAD data.
The crystals used for Se-SAD data collection were grown under similar conditions and appeared in one week.
The Pfu-SRP19 structure (monoclinic form) was solved using a Br-SAD data set collected on a single native crystal briefly soaked in mother liquor supplemented with cryo-protectant and sodium bromide [62].
Crystals of seleno-labeled protein were eventually grown in the same condition obtained for the native protein however they belong to the orthorhombic space group C2221 and despite the collection of a Se-SAD data set on a single crystal, we were unable to solve this structure using the observable anomalous signal.
The nickel sites were located using SHELXD from Bijvoet differences in the Ni-SAD data.
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