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PET imaging with [18F]FEOBV was performed 2 weeks later, and animals were sacrificed the same day for ex vivo immunocytochemistry.
The number of PCNA-IR cells of animals that were sacrificed the same day showed differences among experimental groups [F (4, 25, 9.156, p<0,001].
As it was found for animals that were sacrificed the same day, we were unable to find BrdU/DCX-IR or BrdU/NeuN-IR cells.
Learning the operant conditioning induced a difference in the mean number of BrdU-IR cells between experimental groups that were sacrificed the same day [F (4, 25, 11.97, p<0.001].
A significant difference was found between the mean number of PCNA-IR cell of the experimental groups that were sacrificed the same day [F (4, 25, 13.85), p<0,001] (Figure 2A).
Cage control animals for each PBI group (n = 3) remained undisturbed in their home cages during the behavioral session and were sacrificed the same day and time as their respective exploration group.
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Of course, it's not just our privacy that has been sacrificed: our freedom of speech and our right to due process have been sacrificed by the same laws, and with the same justification, that paved the way to systematic and secret violation of privacy.
Why have America's leaders allowed so many American lives, limbs and dollars to be continuously sacrificed for the same endless mission?
Animals were sacrificed at the same time of day during the course of the study either to comply with animal welfare regulations to avoid unnecessary suffering or at the termination of the study period of 2 years.
As a control, 6 uninfected guinea pigs were sacrificed on the same day as the 30-day infected guinea pigs.
Each control frog was sacrificed at the same time as an infected frog from the same clutch, of the same sex, and of similar size and weight.
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