Sentence examples for sac i from inspiring English sources

Digestion with Sac I resulted in two fragments of 1.5 and 10 kb hybridizing to the probes.

The amplified fragment was digested with Sac I and Xho I, and was then ligated into Sac I/Xho I digested pCC-pgsA.

The amplified fragment was digested with Bam HI and Sac I, and then ligated into Bam HI/Sac I digested plasmid pCC (Tateno et al. 2007b).

After digestion with Sac I, the 6.6 kb fragment was cloned into the corresponding sites in pUC118.

The recombinant expression vectors were then linearized with Sac I and introduced into P. pastoris strain GS115.

The recombinant plasmid pPIC3.5 K-SAM2 linearized with Sac I was introduced into P. pastoris strain GS115 by electroporation method.

The insert of pZ3.51B was then excised as a Sac I- Xba I fragment and cloned into pZ3UIA3A to give plasmid pZ3AM1A.

I had an idea about how to spruce up this bleak vista, replicating a cul-de-sac I once lived on, with a green center.

Finding all of my ideal clients in a cul-de-sac, I sold out and yes, I got my shoes!

One had a Sac I/ Kpn I cloning site and the other a naturally occurring CACC sequence just before the Sac I/ Kpn cloning site (Table 3).

The pMCG161-OsNAP plasmid was digested with Sac I/ Spe I and inserted into the pDS1301-OsNAP to generate the RNAi construct.