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Computational modeling highlighted that key to high enantioselectivity is the discrimination of the R and S substrates by the sole hydrophobic binding pocket based on their size and bulkiness.
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S substrate, P product.
V velocity; S substrate concentration.
ES (enzyme substrate complex) is formed from the reaction between E (enzyme) and S (substrate).
However, concurrent decrease in kinase activity coupled with substrate overexpression causes loss of regulation of essential substrate(s) (Substrate 1) leading to observable growth defects.
The value of k at Cu x S substrate was obtained from the simulated PAC that overlapped the experimental one.
The COMSOL multiphysics software (version 3.4) was employed to investigate the electrical property of the synthesized Cu x S substrate by simulating the probe approach curves (PACs) above the Cu x S substrate [31], which is similar to the work done by Cornut and Lefrou who reported an infinite substrate in the simulation model [32].
Figure 1 General diagram of laccase redox mechanism (SH reduced substrate, S oxidized substrate).
(M, MWNT; S, FTO substrate (SnO2); Z, ZnO).
The mechanism(s) of substrate binding will need to be elucidated in detail.
Moreover, CYP2C93 metabolized the typical human CYP2C9 (diclofenac, flurbiprofen, and tolbutamide) and CYP2C19 (S-mephenytoin) substrates.
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