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Ponceau S solution (Sigma) was used according to the manufacturer's instruction.
But what I'm going to show you here is the 1 s solution.
Thus, this metric measures how satisfied, using the S solution, the user's preferences were.
For the Alizarin red S staining, cells were stained with 2% Alizarin red S solution (Sigma).
Following imaging, membranes were incubated in Ponceau S solution (Sigma, USA), to conform equal loading of protein in each well.
Efficient protein transfer was controlled for by staining with Ponceau S solution and the membranes sequentially incubated with primary and secondary antibodies.
Similar(26)
By contrast, inhibition activity of G-T-S solution group showed less change between 48 h and 24 h.
The OLA-S solution was then injected into the OLA metal precursor solution.
Obtaining the analytic form of the small-S solution still remains our future work.
Staining of cultures was done by incubating in 0.5% alizarin red-S solution for 5 minutes, followed by rinsing with distilled water and air-drying.
Patients were instructed to gently shake the PEG-S solution until the simethicone was dispersed and the solution clear.
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