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The focal mechanism is characterized by using angles of strike (phi) S, dip δ, and rake λ.
The cleaning procedure was finalized with a 35 min plasma asher treatment in oxygen atmosphere and a 10 s dip into diluted hydrochloric acid.
One station located in the inner edge of the anomaly region, named Palmas (10.12° S, 48.21° O, 7.73° S dip lat), and another station located under the southern crest of the anomaly, situated at São José dos Campos (23.07° S, 45.52° O, 19.61° S dip lat).
Observations of the OI 630 nm nightglow emission using a wide-angle imaging system have been carried out at Cachoeira Paulista (22.7° S, 45° W, 15.8° S dip latitude), Brazil during the period 1987 to 1999.
Selection coefficients estimated in this way were s hap = 0.031 ± 0.004 (S.E.M ., against the mutator in haploid populations, and s dip = 0.023 ± 0.002 (S.E.M). in favor of the mutator in diploid populations.
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After photolithographic patterning, the SOG everywhere in the PS was removed by a short 10-s dip in 10% HF/ethanol, which resulted in an as-fabricated PS film selectively covered by photoresist.
Our tests showed a 10-s dip in 10% HF/DI is sufficient to remove all SOG in an exposed PS film (where there was no photoresist) up to 2.45-μm thick.
The L-DIP primers were designed to include a 3′end region that was complementary to the inserted sequence, whereas the S-DIP primer lacked the inserted sequence at the 3′end and passed the insertion point by three to seven nucleotides (Supp. Table S1).
To examine the minimal amount of DNA template required for successful amplification using S-DIP and STR primers as well as L-DIP and STR primers, we amplified serial dilutions of DNA template from individuals heterozygous for the DIP allele, regardless the STR genotype.
Amplification specificity, when using S-DIP and STR primers as well as L-DIP and STR primers, was tested by amplifying 1 ng of DNA template heterozygous for the DIP allele mixed with increasing quantities of a second DNA homozygous for the opposite DIP allele LL and SS respectively (as "informative genotype 2" in Fig. 1A), both DNA were selected irrespective of the genotype of the linked STR.
This was the case when avoiding 1 (Hartigan's dip = 0.03, p = 0.6, failed bimodality test) as well as 2 OB(s) (Hartigan's dip = 0.01, p = 0.2, failed bimodality test).
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