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The hiss is an artificial sound produced by a 1 s burst from an aerosol spray can.
The cells were disrupted via three sequential 60 s burst on a Vortex mixer followed by cooling on ice for 60 s.
The US was a 1 s burst of white noise (10 ms onset and offset ramp, ∼95 dB sound pressure level), delivered via headphones (PX-660 Pro Luxe, Fujikon, Hong-Kong, China).
Finally, the suspension was homogenized by sonication for 10 min (1 s burst and 1 s cooling period) on ice followed by centrifugation at 10,000 g for 20 min at 4°C.
At the end of the procedure, AF/MRT inducibility was tested in all patients using 20 s Burst stimulation at the effective atrial refractory period, but did not initiate any further ablation treatment.
The resulting cellular suspension was then lysed by sonication with one 10 s burst, and the homogenate was used for DNA measurements by the procedure of Labarca and Paigen (1980).
Similar(47)
Gamma-ray bursts (GRBs) are currently divided into short (T90 < 2 s) and long (T90 > 2 s) bursts.
The intensity of Jovian radio emissions usually varies smoothly with time; however, Jupiter periodically emits short and powerful bursts (S bursts), which can outshine all other components.
Cells were lysed by sonication (4 rounds of 30 s bursts on ice).
Cells were lysed by sonication using 10 × 10 s bursts.
The cell suspensions were then sonicated for 10 ×45 s bursts at 4 °C with 30 s intervals.
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