Exact(60)
Oh, and add a fancy S at the end.
Incubate for 40 s at room temperature.
Winds S at 5 to 10 mph.
Winds S at 10 to 15 mph.
The right diuretic(s) at the right dose.
The cycle conditions were: 30 s at 94°C, 30 s at 57°C and 30 s at 72°C.
PCR conditions were 95C for 3 min, followed by 40 cycles consisted of 15 s at 95C, 30 s at 58.7C, and 30 s at 72C for Slug; 95C for 3 min, followed by 40 cycles consisted of 15 s at 95C, 30 s at 53.6C, and 30 s at 72C for E-cadherin; 95C for 3 min, followed by 40 cycles consisted of 15 s at 95C, 30 s at 62C, and 30 s at 72C for PSMB6.
After denaturation (95°C for 2 min), DNA was amplified by 25 cycles (30 s at 95°C, 30 s at 55°C, 30 s at 72°C).
PCRs were performed by running 30 cycles with a temperature profile of 30 s at 95°C, 30 s at 57°C, and 60 s at 72°C.
The PCRs were run using the following conditions: 30 s at 94°C, 30 s at 50°C, 30 s at 72°C (35 cycles).
Conditions used were as follows: denaturation, 30 s at 94°C; annealing, 30 s at 48°C; extension, 60 s at 72°C; 30 cycles.
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