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As a negative control, experiments were performed with ultrasound treated (20 s, amplitude 50%%) mitochondria to exclude interference of dye and membrane fragments, as well as with IFM to confirm Cx43 hemichannel-specific effects.
The suspensions were sonicated (three cycles of 15 s, amplitude 30%) and centrifuged at 16,100 g for 10 min.
Table 1 Model parameters Parameters Value Wave Period T p 2.5 s Amplitude H 0.35 m Float Radius R float 1 m Mass m float 1680 kg Height H float 0.7 m Counterweight Mass m M 150 kg Driving pulley Radius R 0.18 m Gearbox Gear ratio k 10.15 Seawater Density ρ 1028 kg/m3.
The sonicate was prepared by an initial trypsination of confluent cell culture flasks followed by sonication for 30 s (amplitude 10 microns) using a MSE Soniprep 150 sonicator (Sanyo).
For this, fifty mg of the sampled tissues were homogenised by sonication for 30 s (amplitude 10 microns) using a MSE Soniprep 150 sonicator (Sanyo) and RNA was further isolated using a GenElute Mammalian™ Total RNA Miniprep Kit (Sigma).
Current-clamp recordings were made by an Axoclamp 2B amplifier (Molecular Devices Corp., Sunnyvale, CA, USA) and intrinsic firing properties were investigated by injecting step-depolarizing currents (duration 1 s, amplitude ±10 200 pA).
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We employed two forms of EDR: QRS area summation (EDR_G) and R-S amplitude tracking (EDR_RS) [4].
Results: Surface electromyogram values and S-MUAP amplitudes increased during both 10%and30%0% MVC fatiguing contractions, while mean firing rates decreased.
A sine-like tilt trajectory with a period of 1 s and amplitude of ±15° was used.
S N (Wöhler) curves of stress amplitude (σa = S) versus cycles to failure (N) are presented.
To estimate the relative magnitude of each event, the ratio of the maximum S-wave amplitude of each of the latter two events to the S-wave amplitude of Event 1 was calculated for each F-net station.
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