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At Loaf, a new bakery and cafe, an assortment of breads — ciabatta, dill, whole wheat, raisin, foccacia and rye — line a high wooden shelf, their crusty good looks set off by a vibrant backdrop of buttercup-yellow paint.
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A shorter plant height is a key agronomic property that distinguishes modern rye lines from older breeding material.
Moreover, pedigree information can be an insufficient criterion to conclude about genetic relationships between rye lines obtained using selfing.
The rye genes absent or unexpressed in triticale could be accounted for by gene deletion after polyploidization or by their absence in the rye lines that were parental to the triticale lines used in the study.
In total, 7,639 bp from eleven candidate genes in 201 rye lines were amplified resulting in 147 SNPs, nine Indels, and an average SNP frequency of 1 SNP/52 bp (Table 1).
A rye RNAi line (#11392) from the VDRC stock center in Vienna was crossed into the ryeP-GAL4 line along with a UAS-Dicer2 transgene, included to improve efficacy of the RNAi.
Accordingly, [ 36] detected 11 genomic regions significantly contributing to plant height among testcrosses with two rye introgression line libraries.
However, the remaining 12.21% revealed either pronounced sequence differences (highly polymorphic genes/alleles) or genes that are represented (expressed) in only one of the five rye inbred line samples.
In a pilot experiment, we analyzed 59 rye inbred lines including the five lines used for sequencing with the Rye5K SNP array to estimate the degree of residual heterozygosity.
Performance of the Rye5K SNP array was investigated by genotyping 59 rye inbred lines including the five lines used for sequencing, and five barley, three wheat, and two triticale accessions.
Using molecular and cytological approaches in a wheat-rye addition line (wheat genome plus the rye nucleolar chromosome pair 1R), we investigated transcriptional activity and chromatin topology of the wheat dominant NORs in a nucleolar dominance situation.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com